Robert De Niro and his wife Grace Hightower shared after more than 20 years of marriage, according to US media reports.
The couple live separately for a while, said The Hollywood Reporter, the celebrity website TMZ and People magazine, citing anonymous sources.
The star of the film, including Kuma II, Raging Bull, Taxi Driver, Cape Fear, Casino and Goodfellas, and the winner of two Oscars, De Niro he carefully kept his family and private life out of the center of attention.
"Sometimes things do not work the way you hope or want them to be," said a source who was close to the family, he says in people.
The six pages, which reported the break-up for the first time, say a couple are expected to be publicly publicized in the coming weeks.
De Niro, 75, married Hightower, 63, 1997 after they met at the London restaurant. They have two children – Elliot, 20, and six-year-old Helen Grace.
This is not their first break-up, and the actor signed up for the 1999 divorce. They later reunited and renewed their wedding vows in 2004.
Hightower is the other woman De Niro. He was married to Dick Abbott from 1976 to 1988 and had two children.
The star also has two children with former girlfriend Toukie Smith.
His spokesperson did not return the requests for a comment on the division.
De Niro found himself in the headlines last month when he was among the goals of the crowds of suspicious packages who were sent in post to critics of Donald Trump in the United States.
After the bomber unit was forced to remove a packet directed by an actor from his office in Manhattan, New York, he issued a statement inviting people to vote in mid-term elections.
"There is something stronger than a bomb," he said, "and that's your voice. People have to vote!
"Thank God that no one is hurt, and I thank the brave and resourceful staff of security and law to protect us."
De Niro was an open critic of Mr. Trumpa, who declared at one stage he would want to hit the president in the face.