Scientists have developed a pioneering new technique that could revolutionize the accuracy, precision, and clarity of superresolution imaging systems.
A team of scientists, led by Dr. Christian Soeller of the University of Exeter, Institute of Living Systems, which supports interdisciplinary research and is the center of new high-resolution measurement techniques, has developed a new way to improve very fine, molecular images of biological samples.
The new method builds on the success of an existing super-resolution imaging technique called DNA-PAINT (accumulation of a recording point in nanoscale topography) – where molecules in a cell are marked with marker molecules attached to individual DNA strands.
The corresponding DNA strands are then also labeled with a fluorescent chemical compound and introduced into the solution – when they bind marker molecules, a ‘flickering effect’ is created that allows imaging.
However, DNA-PAINT in its current form has a number of disadvantages that limit the applicability and performance of the technology when imaging biological cells and tissues.
In response, the research team developed a new technique, called DNA Dye Repetition, that is capable of suppressing background noise and nonspecific signals, as well as reducing the time required for the sampling process.
Crucially, the use of Repeat DNA-PAINT is simple and has no known drawbacks, it is routinely applicable, consolidating the role of DNA-PAINT as one of the most powerful and versatile methods of molecular resolution.
The study was published in Natural communications January 21, 2021
Dr. Soeller, lead author of the study and biophysicist at the Institute of Living Systems, said: “Molecular details can now be seen by light microscopy in a way that seemed unattainable a few years ago. This allows us to see directly how molecules orchestrate complex biological functions diseases. “
The research was made possible by colleagues from physics, biology, medicine, mathematics and chemistry working together across traditional discipline boundaries. Dr. Lorenzo Di Michele, co-author with Imperial College London, said: “This work is a clear example of how quantitative biophysical techniques and concepts can really improve our ability to study biological systems.”
Breakthrough images in super resolution in living cells
Alexander H. Clowsley et al., DNA-PAINT repetition suppresses background and nonspecific signals in optical nanoscopy, Natural communications (2021). DOI: 10.1038 / s41467-020-20686-z
Provided by the University of Exeter
Quote: A pioneering new technique could revolutionize super-resolution image processing systems (2021, January 21) downloaded January 21, 2021 from https://phys.org/news/2021-01-technique-revolutionise-super-resolution -imaging.html
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